This study shows that PKR binds tightly and specifically to ATP (B) but not to CTP, GTP, or UTP (A).
|ATP into PKR (B)|
|Kd = 19.0 µM|
This is important for identifying the number of triphosphate binding sites and their locations on PKR, which relates to its function in innate immunity.
We used auto-ITC to measure the thermodynamic parameters for NTP binding to PKR.