The Huck Institutes of the Life Sciences

Primers supplied

Sequencing primers supplied by the facility free of charge

Sequencing primers

  • T7 5’TAA TAC GAC TCA CTA TAG GG 3’
  • T7T 5'GCT AGT TAT TGC TCA GCG G 3’
  • T3 5’ATT AAC CCT CAC TAA AGG GA 3’
  • SP6 5’CGA TTT AGG TGA CAC TAT AG 3’
  • M13Universal, M13-21 5’ TGT AAA ACG ACG GCC AGT 3’
  • M13-40 5’GTT TTC CCA GTC ACG AC 3’
  • M13-47 5’CGC CAG GGT TTT CCC AGT C 3’
  • M13Rev 5’ CAG GAA ACA GCT ATG ACC 3’
  • M13R-48 5’ AGC GGA TAA CAA TTT CAC A 3’
  • GAL4AD TAC CAC TAC AAT GGA TG
  • GAL4BD TCA TCG GAA GAG AGT AG

Homopolymer Primers for sequencing strings of nucleotides

  • PolydT (21T’s) C PolydT (21T's) A, C,G as the last base -- mixture.
  • PolydA (20A’s) T