The Huck Institutes of the Life Sciences

Applied Biosystems 7900HT Fast Real-Time PCR System

Model #: Applied Biosystems 7900HT Fast Real-Time PCR System

 

The acknowledged gold standard in real-time PCR

Applied Biosystems 7900HT Fast Real-Time PCR System

Applied Biosystems 7900HT Fast Real-Time PCR System

Related information

Considerations for real-time qPCR sequencing
Things you need to be aware of when planning a real-time qPCR analysis at the Genomics Core Facility

 

Uses

  • Gene expression quantification
  • SNP determination
  • Copy number variation

 

Specifications

  • 384-well peltier-based block
  • Calibrated for 4 dyes
  • Inclusion of ROX in the master mix normalizes wells to each other
  • 10-20 µl volume for standard gene expression assays
  • As little as 5 µl can be used for SNP determination assays, saving on cost
  • Low-density array cards can also be used

 

Details

  • Applied Biosystems Twister Autoloader allows multiple plates to be loaded automatically.
  • Reserve system for use on the Core Cal via dnaLIMS.

 

Related services

RNA and DNA samples can also be quantified by the Facility, using Taqman assays from nucleic acid by absolute or relative quantification.

 

Related instruments

  • Applied Biosystems Twister Autoloader

 

Equipment Location

Genomics Core Facility