The Huck Institutes of the Life Sciences

Sanger sequencing

Information about Sanger sequencing services provided by the Genomics Core Facility at University Park

 

Services

  • Regular sequencing: standard plasmid and PCR products as well as BACs and cosmids
  • High-throughput sequencing: 96-well plate submissions

 

Specifications

    Please inquire regarding specifications for submitting plates before submitting them.

     

    Requests

      All sequencing requests must be made using dnaLIMS.

      If you bring us DNA of less than 10K by 9am, your sequence should typically be ready to download by 5pm.

      However, 96-well plates are not guaranteed to be set up the same day we receive them, so results can take slightly longer.

       

      Data analysis

      Please note: the facility does not provide data analysis services.

       

      Sanger Sequencing Presentation

         

        Instrumentation

        Applied Biosystems 3730XL