- 4 lasers (405, 488, 543, 633 nm) plus 405nm SIMS for FRAP
- Time controller for complex experiments
- Olympus IX70 inverted microscope with fluorescence burner
- UplanAPO 10X/0.40, UPlanFL 20X/0.50, UAPO/340 40x/1.50 water, UPlanFL 40X/1.3 oil, PlanApo 60X/1.4oil, UPlanSApo 100X/1.4 oil
Four single-line lasers with individual shutters that are software-controlled for sequential acquisition.
- Violet (405nm, 10mW)
- Blue argon (488nm, 10mW)
- Green HeNe (543nm, 10mW)
- Red HeNe (633nm, 10mW)
- Scanning modes:
- 1-dimensional (spot-scan), 2-dimensional (X-Y vector, X-Z, or time)
- 3-dimensional (X-Y-Z, or X-Y-time) or 4 dimensional (X-Y-Z-time)
- Transmitted light:
- External halogen light source connected to microscope via fiber cables, external PMT for light collection.
- DIC optics are available for all objectives.
- Confocal LSM improves the resolution of images by recording fluorescence or reflected light when a laser is focused at set focal planes in tissues and cells, rejecting all other light coming from planes above or below the one of interest. This improves resolution of sub-micron structures within fixed and live cells and tissues. A series of images can be recorded axially and either analyzed separately or incorporated into a composite image.
- SIM Scanning:
- Simultaneous photobleaching and scanning.
- Excellent for rapid FRAP or acceptor pFRET.
- Spectral imaging:
- Using either established fluorescence spectra or blind spectral deconvolution Time controller: enables multiple diverse imaging conditions to run with automatic controls
- Fluoview: captures files as MultiTIFF files (*.oib) and displays as TIFF files (*.tiff)