Model: Olympus Fluoview 300
A basic confocal laser scanning microscope requiring manual filter and laser set-ups, and capable of highly sensitive and sophisticated multi-color imaging
- Blue, red and green lasers for multi-color imaging
- X, Y, Z scanning for 3-D reconstruction
- Olympus IX70 inverted microscope with fluorescence burner
- Uplan4X, UplanFL 10X/0.30, planFL 20X/0.50, UplanFL 40X/0.75, PlanApo 60X/1.4oil, Plan Apo 100X/1.3
Three single-line lasers with individual shutters that are software-controlled for sequential acquisition.
- Blue argon (488nm, 10mW)
- Green HeNe (543nm, 10mW)
- Red HeNe (633nm, 10mW)
- Scanning modes:
- 1-dimensional (spot-scan)
- 2-dimensional (X-Y vector, X-Z, or time)
- 3-dimensional (X-Y-Z, or X-Y-time)
- 4 dimensional (X-Y-Z-time)
- Transmitted light:
- External halogen light source connected to microscope via fiber cables, external PMT for light collection.
- DIC optics are available for all objectives.
- Confocal LSM improves the resolution of images by recording fluorescence or reflected light when a laser is focused at set focal planes in tissues and cells, rejecting all other light coming from planes above or below the one of interest. This improves resolution of sub-micron structures within fixed and live cells and tissues. A series of images can be recorded axially and either analyzed separately or incorporated into a composite image.
- Fluoview: captures files as MultiTIFF files (*.oib) and displays as TIFF files (*.tiff)