The Huck Institutes of the Life Sciences

Gene-targeted mouse production

Production of targeted mutant mice by embryonic stem cell microinjection

What you will need

Investigators should have:

  • A PSU IACUC approved protocol so that chimeric progeny can be transferred to your protocols at weaning
  • Consulted with the Animal Resources Program to acquire mouse space in one of the PSU campus facilities
  • Evidence of a screening assay (either PCR or Southern), for your mutant allele, so you can detect germline transmission
  • Investigators who want to supply their own targeted embryonic stem cell line must have commercial documentation that the cell line was MAP tested for murine adventitious agents prior to expansion